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Record the serial numbers of the pipeters you use (if they have serial numbers) else label the pipeters with tape and marker.
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What do you need to know in order to make a dilution which will meet the needs of the situation?) (Suppose you are in a research lab and you need to make a dilution of a concentrated reagent stored in the refrigerator.
- What do you need to know? Before making your dilution, make a list of things you need to know, and check it with your instructors.
- Make a dilution which reduces the concentration by 37-fold, which is a 1/37 dilution. You are provided with a concentrated stock solution of a yellow solute (p-nitrophenol), labeled 'NP Stock'. It is important to know how to design and perform dilutions which are accurate and which meet the needs of the situation. Reagent dilutions are needed daily in biochemical or immunological lab work.
- Verify instrument performance with simple tests.
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- Pros and cons of conventional spectrophotometer vs.ĩ6-well plate absorbance reader.
- Determine protein concentration with spectrophotometer.
- Determine precision and accuracy of dilutions.
- Understand how to calculate optimal dilutions.
- Spectrophotometry & Dilutions Microbiology 542 - Eric Martz Goals: 1 ml added to 9 ml gives a 10-fold dilution 1 ml added to 99ml. Then you just have to take one part (0.4mL) OS and 4 parts (1.6mL) solvent and you have your finished sample.Serial dilution involves repeatedly mixing known amounts of source culture with (sterilised) liquid. 2mL/5 = 0.4 mL So 0.4mL represents one part.
For example for a concentration of of 1/5 you need 1 part OS and 4 parts solvent, so the most rational way of mixing those up and ending up with 2mL is to divide the intended 2mL by 5. To always end up with 2mL of your diluted samples you just have to take a look at how many parts of the OS and solvent you need. For a concentration of 1/10 it´s exactly the same. 20mM, then you have to take one part of your OS and add 4 parts of pure solvent (third column 0.4mL : 1.6mL). Now if you want a concentration of 1/5, i.e. 50mM, you take one half of your OS and add one half of pure solvent (second column 1mL : 1mL). So for a concentration of 1, in your case 100mM, you obviously just take the OS. You want one with a concentration of 1 (relative to your original concentration), one with a concentration of 1/2, one with 1/5 and one with 1/10 - of course all derived from your original solution (OS). OK, so you want to dilute your original solution in different ways.